Device

Part:BBa_K3740030:Design

Designed by: Guiyi Huang   Group: iGEM21_SZPT-CHINA   (2021-09-20)


J23100-B0034-bphS-pET RBS-bphO-rrnB T1-J23109-B0034-fcsR-rrnB T1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2818
    Illegal NheI site found at 2841
    Illegal NotI site found at 189
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 294
    Illegal BglII site found at 1357
    Illegal XhoI site found at 632
    Illegal XhoI site found at 2489
    Illegal XhoI site found at 3577
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 447
    Illegal NgoMIV site found at 621
    Illegal NgoMIV site found at 938
    Illegal NgoMIV site found at 999
    Illegal NgoMIV site found at 2678
    Illegal NgoMIV site found at 2935
    Illegal NgoMIV site found at 3680
    Illegal AgeI site found at 2634
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 982
    Illegal BsaI site found at 3574
    Illegal BsaI.rc site found at 482
    Illegal BsaI.rc site found at 1456
    Illegal BsaI.rc site found at 3322
    Illegal SapI.rc site found at 3687


Design Notes

None


Source

J23100-BphS-BphO

References